When use of a flow cell is undesirable, Wyatt offers a microCuvette Adapter Kit which replaces the flow cell of a DAWN with a high-quality, low-volume microcuvette. Historically, batch measurements have been laborious and prone to operator error the Calypso ® automates batch MALS for accurate, reliable and rapid results. For maximum accuracy the measurement is carried out over several concentrations. These unfractionated, or 'batch', measurements are typically carried out via direct injection into a MALS flow cell. B 22), a measure of solute-solute interactions. Analysis of unfractionated samples yields the weight-average molecular weight M w of the solution, as well as the second virial coefficient A 2 (a.k.a. See the SEC-MALS and FFF-MALS pages to learn how these versatile techniques combine multiple detectors, including theĪSTRA ® software in order to extract a wealth of information about your samples beyond molar mass.Įven if samples are not compatible with a separation technique, MALS can still be used to perform analysis of molar mass. Stoichiometry of tightly bound protein heterocomplexes.Accurate aggregation and oligomeric states of proteins, peptides and virus-like particles (VLPs).

Molar mass distributions of heterogeneous samples such as carbohydrates or synthetic polymers.

Important Applications of SEC-MALS and FFF-MALS molar mass in order to provide accurate molar mass distributions across the chromatogram or fractogram. Incorporation of independent, absolute MALS analysis eliminates reliance on unreliable column calibration, retention time and models of hydrodynamic size vs. MALS detectors are most commonly integrated with a separation technique such as size exclusion chromatography

Dynamic & Electrophoretic Light Scattering.

Molar mass calculator